diff --git a/cmd/web_acme.go b/cmd/web_acme.go
index 10a00cc4ec5..766ec56c2e4 100644
--- a/cmd/web_acme.go
+++ b/cmd/web_acme.go
@@ -83,6 +83,7 @@ func runACME(listenAddr string, m http.Handler) error {
 		TrustedRoots:            certPool,
 		Email:                   setting.AcmeEmail,
 		Agreed:                  setting.AcmeTOS,
+		Profile:                 setting.AcmeProfile,
 		DisableHTTPChallenge:    !enableHTTPChallenge,
 		DisableTLSALPNChallenge: !enableTLSALPNChallenge,
 		ListenHost:              setting.HTTPAddr,
diff --git a/modules/setting/server.go b/modules/setting/server.go
index 641e18bdb4c..cb91a900e24 100644
--- a/modules/setting/server.go
+++ b/modules/setting/server.go
@@ -100,6 +100,7 @@ var (
 	AcmeLiveDirectory          string
 	AcmeEmail                  string
 	AcmeURL                    string
+	AcmeProfile                string
 	AcmeCARoot                 string
 	SSLMinimumVersion          string
 	SSLMaximumVersion          string
@@ -142,6 +143,7 @@ func loadServerFrom(rootCfg ConfigProvider) {
 		Protocol = HTTPS
 		if EnableAcme {
 			AcmeURL = sec.Key("ACME_URL").MustString("")
+			AcmeProfile = sec.Key("ACME_PROFILE").MustString("")
 			AcmeCARoot = sec.Key("ACME_CA_ROOT").MustString("")
 
 			if sec.HasKey("ACME_ACCEPTTOS") {
diff --git a/modules/setting/server_test.go b/modules/setting/server_test.go
new file mode 100644
index 00000000000..cb36f9dc806
--- /dev/null
+++ b/modules/setting/server_test.go
@@ -0,0 +1,39 @@
+// Copyright 2026 The Gitea Authors. All rights reserved.
+// SPDX-License-Identifier: MIT
+
+package setting
+
+import (
+	"testing"
+
+	"gitea.dev/modules/test"
+
+	"github.com/stretchr/testify/assert"
+)
+
+func TestLoadServerFromACMEProfile(t *testing.T) {
+	defer test.MockVariableValue(&Protocol)()
+	defer test.MockVariableValue(&EnableAcme)()
+	defer test.MockVariableValue(&AcmeTOS)()
+	defer test.MockVariableValue(&AcmeURL)()
+	defer test.MockVariableValue(&AcmeProfile)()
+	defer test.MockVariableValue(&AcmeCARoot)()
+	defer test.MockVariableValue(&AcmeLiveDirectory)()
+	defer test.MockVariableValue(&AcmeEmail)()
+
+	cfg, err := NewConfigProviderFromData(`
+[server]
+PROTOCOL = https
+ENABLE_ACME = true
+ACME_ACCEPTTOS = true
+ACME_DIRECTORY = /tmp/acme
+ACME_EMAIL = acme@example.com
+ACME_PROFILE = shortlived
+`)
+	assert.NoError(t, err)
+
+	loadServerFrom(cfg)
+
+	assert.Equal(t, HTTPS, Protocol)
+	assert.Equal(t, "shortlived", AcmeProfile)
+}